| 2 | From 3 August | The tram service will be fully replaced by buses. | All day | TOWARDS PIAZZA MANCINI - At Piazzale Flaminio, buses depart from bus line 490 stop.
- At Piazza della Marina, buses stop on the side lane.
- At Piazzale delle Belle Arti, buses use the tram stop.
- On Viale Tiziano, buses stop on the side lane, sharing the stop with bus line N201.
- Instead of stopping on Viale Pinturicchio, buses stop on Lungotevere Thaon di Revel.
TOWARDS PIAZZALE FLAMINIO - At Piazza Mancini, buses depart from the bus terminus.
- Instead of stopping on Via Masaccio, buses stop on Via Flaminia near Piazza dei Carracci, sharing the stop with bus line N201.
- On Via Flaminia, buses use the side-lane stop shared with bus line N201.
- At Piazzale delle Belle Arti and Piazza della Marina, buses use the tram stops.
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| 3 | From 7 September
| The tram service operates between Porta Maggiore and Trastevere Station. Between Porta Maggiore and Valle Giulia, the service is replaced by buses.
| All day | TOWARDS PORTA MAGGIORE - Buses follow the normal tram route in the tram lane as far as Viale dello Scalo di San Lorenzo.
- At Piazza di Porta Maggiore, buses terminate in the centre of the square.
TOWARDS VALLE GIULIA - Buses follow the tram route as far as Viale Rossini, then run via Via Bertoloni, Piazza Pitagora, Viale Bruno Buozzi, Viale delle Belle Arti, Viale Tiziano, Via Fratelli Archibugi, Via Flaminia and Viale delle Belle Arti, terminating at Valle Giulia.
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| 3 | BETWEEN PORTA MAGGIORE AND TRASTEVERE STATION From 21 to 25 September, due to works in preparation for the operation of the new CAF Urbos trams, | All services departing from Trastevere from 23:33 onwards are replaced by buses along the entire route. All services departing from Porta Maggiore from 23:11 onwards are replaced by buses. Bus services arriving at Porta Maggiore from Valle Giulia after 23:10 continue to Trastevere Station | late evening service | Replacement buses operate as follows: TOWARDS PORTA MAGGIORE - Up to Via Marmorata, buses make the same stops as the tram.
- At Piazzale Ostiense, buses stop on the side lane of Viale della Piramide Cestia.
- Buses then use the side lanes of Viale della Piramide Cestia, Piazza Albania and Viale Aventino, followed by Piazza di Porta Capena, Via di San Gregorio, Via Celio Vibenna and Piazza del Colosseo.
- From Via Labicana, buses follow the normal tram route.
TOWARDS TRASTEVERE STATION - Up to Piazza del Colosseo, buses make the same stops as the tram.
- Buses then run via Via Celio Vibenna, Via di San Gregorio, the side lanes of Piazza di Porta Capena, Viale Aventino and Viale della Piramide Cestia.
- At Piazzale Ostiense, buses stop at the end of Viale della Piramide Cestia, near the park.
- From Via Marmorata to the Ministry of Education, buses make the same stops as the tram, then stop on the side lane of Viale Trastevere.
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| 5 | From 28 August due to track renewal works on Via Mann/Via Farini | The tram service will be fully replaced by buses. | All day | Buses serve the same stops as the tram except: TOWARDS PIAZZA DEI GERANI - Buses run via Viale Enrico de Nicola, Piazza dei Cinquecento (inner terminus area), Via Cavour and Via Farini.
- At Porta Maggiore, buses stop on the side lane after Via di Porta Maggiore (stop 71299).
- At Piazzale Labicano, buses stop on Via Prenestina after the traffic lights.
- Along Via Prenestina, between the depot and Largo Preneste, buses use the side lanes, following the route of night bus N5.
TOWARDS TERMINI STATION - Along Via Prenestina, between Largo Preneste and the depot, buses use the side lanes, following night bus N5.
- At Porta Maggiore, buses stop on the side lane before Via di Porta Maggiore (stop 76628).
- From Via Napoleone III, buses run via Via Gioberti and Via Giolitti, terminating at Piazza dei Cinquecento (outer parking area).
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| 8 | From 21 September | The tram service operates along the entire route between Casaletto and Piazza Venezia. From 21 to 25 September, some evening services are replaced by buses due to works in preparation for the operation of the new CAF Urbos trams. | evening service | TOWARDS PIAZZA VENEZIA All services departing from Casaletto from 22:56 onwards are replaced by buses. - At Casaletto, buses depart from the roadside terminus shared with bus line 088.
- On Circonvallazione Gianicolense, buses stop on the side lane at the same stops as night bus N8.
- From Viale Trastevere to Via Arenula, buses serve the same stops as the tram.
TOWARDS CASALETTO All services departing from Piazza Venezia from 22:47 onwards are replaced by buses. - The Piazza Venezia terminus is relocated to Via degli Astalli.
- On Via Arenula, buses serve the same stops as the tram.
- On Viale Trastevere and Circonvallazione Gianicolense, buses use the side lane and stop at the same stops as night bus N8.
- Buses also stop at Largo Argentina.
Some evening services between Casaletto and Porta Maggiore may also be replaced by buses.
Buses serve the same stops as the tram except: TOWARDS TOGLIATTI - Buses run via Viale Enrico de Nicola, Piazza dei Cinquecento (inner terminus area), Via Cavour and Via Farini.
- At Porta Maggiore, buses stop on the side lane after Via di Porta Maggiore (stop 71299).
- At Piazzale Labicano, buses stop on Via Prenestina after the traffic lights.
- Along Via Prenestina, between the depot and Largo Preneste and between Via Bresadola and Viale Palmiro Togliatti, buses use the side lanes as night bus N5.
- On Viale Palmiro Togliatti, buses use the side lanes.
TOWARDS TERMINI STATION - On Viale Palmiro Togliatti, buses use the side lanes.
- Along Via Prenestina, between Viale Palmiro Togliatti and Via Bresadola and between Largo Preneste and the depot, buses use the side lanes as night bus N5.
- At Porta Maggiore, buses stop on the side lane before Via di Porta Maggiore (stop 76628).
- Buses from Via Napoleone III will run via Via Gioberti and terminate at Piazza dei Cinquecento (outer parking area).
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| 14 | From 28 August due to track renewal works on Via Mann/Via Farini | The tram service will be fully replaced by buses. | All day |
| 19 | From 7 September | The tram service will be fully replaced by buses. | All day | Buses serve the same stops as the tram except: TOWARDS VIALE GIULIO CESARE/Ottaviano - Along Via Prenestina, between Largo Preneste and the depot, buses use the side lane, following the route of night bus N5.
- From Viale Gioacchino Rossini, buses run via Via Bertoloni, Piazza Pitagora, Viale Bruno Buozzi, Viale delle Belle Arti and then follow the normal route.
TOWARDS PIAZZA DEI GERANI - From Viale Giulio Cesare, buses run via Viale delle Milizie, Lungotevere, Piazzale delle Belle Arti, Viale Bruno Buozzi, Piazza Pitagora, Via Antonio Stoppani, Viale Parioli, Viale Liegi, then follow the normal tram route as far as Viale dello Scalo di San Lorenzo.
- Along Via Prenestina, between the depot and Largo Preneste, buses use the side lane, following the route of night bus N5.
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